摘要
利用RT PCR方法分析了生长于冷杉木片上的黄孢原毛平革菌木质素过氧化物酶基因lipA2 (GLG3)、lipC1 (GLG2 )、lipC2 (GLG5 )、lipD2 (GLG1 )、lipE(LP0 81 1 )的表达。结果发现在不同的培养时间里仅有特定的基因表达 ,在第 2周时仅有lipA2 (GLG3)基因表达 ,在第 4周时未检测到任何基因的表达 ,在第 6周时lipD2 (GLG1 )和lipC1 (GLG2 )基因表达 ,在第 8周时仅有lipA2 (GLG3)基因表达。这些结果说明 ,在冷杉木片上培养的黄孢原毛平革菌的lip基因表达具有明显的时间特异性 ,并且与限定培养基中得到的结果明显不同。
Expression of Phanerochaete chrysosporium lipA2(GLG3)、lipC1(GLG2)、lipC2(GLG5)、lipD2(GLG1)、lipE(LP0811) genes were analyzed by RT-PCR method. It was showed that some genes were expressed in special colonized period. Only lipA2(GLG3)transcription occured in the 2nd week and the 8th week, both lipC1(GLG2) and lipD2(GLG1)gene transcription was checked out in the period of 6 weeks. However, no lip genes were expressed in the time of 4 weeks. These results indicated that lip gene expression is relied on the colonized period and transcript patterns are re dramatically different from those in previous studies with defined media.
出处
《微生物学报》
CAS
CSCD
北大核心
2003年第1期65-72,共8页
Acta Microbiologica Sinica
基金
自然科学基金资助项目 ( 30 1 70 5 1 3)
教育部博士基金资助项目 ( 2 0 0 1 0 61 0 0 2 5 )
