摘要
用大肠杆菌克隆载体ｐＵＣ１０构建白腐丝状担子真菌黄孢平革霉（Ｐｈａｎｅｒｏｃｈａｅｔｅｃｈｒｙｓｏｓｐｏｒｉｕｍ）ＭＥ４４６基因组文库，用分离自ＢＫＭ－Ｆ１７６７菌株的木质素过氧化物酶ｃＤＮＡ片段ＣＬＧ４和ＣＬＧ５为探针，从构建的基因组文库中分离到一个含木质素过氧化物酶基因的重组子ｐＧＬＧ－Ｍ１。对该重组子插入片段所作的限制酶谱分析结果表明，它与来自ＢＫＭ－Ｆ１７６７的ＧＬＧ２片段的限制酶谱十分相似。
A genomic library of a filamentous white-rot fungus basidiomycete Phanerochaete chrgsosporium ME446 was constructed in the Bam HI site of Escherichia coli vector pUC 19 and screened with the lignin peroxidase(LIP)-encoding cDNAs:CLG 4 and CLG 5 isolated from strain BKM-F1767 as mixed probes. A positive clone,designated pGLG-M1,was obtained in this study. Restriction mapping of the LIP-encoding fragment indicated that it had a restriction map very similar to that of of GLG2 which wsa isolated from another strain BKM-F1767.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
1994年第2期259-263,共5页
Journal of Sichuan University(Natural Science Edition)
基金
国家自然科学基金
关键词
黄孢平革霉
木质素
过氧化物酶
molecular cloning,genomic library,cDNA probe,restriction mapping.
