摘要
目的:探讨百草枯致小鼠黑质细胞死亡的方式及神经毒性机制。方法:C57BL小鼠口服百草枯不同时间后采用酪氨酸羟化酶(TH)免疫组化及TUNEL法、流式细胞仪、透射电镜检测黑质部多巴胺能神经元的凋亡现象。结果:TH免疫组化及TUNEL双标染色检查示C57BL小鼠暴露于百草枯后2周后黑质部多巴胺能神经细胞即有凋亡样改变,流式细胞仪检测显示小鼠于暴露百草枯1个月时凋亡细胞数最多,与对照组相比,P<0.001;2个月时有所减少,但仍多于对照组(P<0.01)。结论:小鼠暴露于百草枯一定时间后可引起黑质部多巴胺能神经元发生凋亡,这可能是百草枯的神经毒性作用机制之一。
Aim: To investigate the death mode of substantia nigra dopaminergic neurons in C57BL mice exposed to paraquat. Methods: TdT-mediated biotinylated-dUTP nick end labeling (TUNEL)method and tyrosine hydroxylase (TH) immunohistochemistry, flow cytometry and electronic microscopy were performed to detect apoptosis of substantia nigra dopaminergic neurons in mice exposed orally to paraquat for different periods. Results :TH immunohistochemistry and TUNEL method showed apotoric neurons in substantia nigra after C57BL mice exposed to paraquat by orally administration for 2 weeks, apoptotic neurons were most when mice exposed for 1 month, compared with the control, P < 0.001; and were decreased when mice exposed to paraquat for 2 months, but still more than the control, P < 0.01.Conclusion: Paraquat could induce apoptosis of substantia nigra dopaminergic neurons when C57BL mice exposed to paraquat for a relative long period.
出处
《中国临床神经科学》
2003年第1期53-56,共4页
Chinese Journal of Clinical Neurosciences
基金
上海市科学技术发展基金(No.00411905)
关键词
百草枯
黑质细胞
凋亡
小鼠
帕金森病
毒性
paraquat substantia nigra neuron apoptosis mouse Parkinson disease
