摘要
目的 探讨乙肝病毒、丙肝病毒感染在肝门部胆管癌发病中的作用及与 p5 3异常表达的关系。方法 采用免疫组织化学方法对 6 8例石蜡包埋肝门部胆管癌标本中的HBV X、HCV C和p5 3蛋白进行检测 ,并结合临床资料进行分析。 结果 6 8例肝门部胆管癌中乙肝病毒X蛋白阳性率为 8 8% (6 / 6 8) ,丙肝病毒C蛋白阳性率为 35 % (2 4 / 6 8) ,两者均阳性 1例 (1 5 % ) ;p5 3蛋白阳性率为4 5 6 % (31/ 6 8)。乙肝、丙肝病毒感染的肝门部胆管癌在分化和浸润程度、淋巴结转移、根治程度与非病毒感染的肝门部胆管癌有明显差异 ,p5 3与乙肝病毒X、丙肝病毒C蛋白表达呈显著的正相关 (P <0 0 0 1)。结论 乙肝、丙肝病毒感染与肝门部胆管癌发生有关。乙肝、丙肝病毒感染的肝门部胆管癌恶性程度高 ,可能有较差的预后。HBV X、HCV C蛋白可能在HBV、HCV感染肝门部胆管癌的病因中起重要作用 ,p5 3蛋白在肝门部胆管癌中的异常表达与HBV、HCV感染有关。
Objective To study the relationship between the infection of HB(C)V and p53 in human hilar cholangiocarcinoma. Methods Immunohistochemistry was used to detect HBxAg, HCV C and p53 protein in 68 formalin fixed and paraffin embedded samples of hilar cholangiocarcinoma. Meanwhile, the clinical data of the patients were analyzed. Results Six cases (8 8%) were positive for HBxAg and 24 (35%) for HCV C protein. One case was positive for both HBxAg and HCV C protein. Thirty one cases (45 6%) were positive for p53 protein. There were statistical differences in extent of differentiation, invasion and lymph node metastasis between the patients with HB(C)V infection and those without the infection. The positive correlation of HBxAg/HCV C protein and p53 in the patients with hilar cholangiocarcinoma was confirmed. Conclusions The infection of HB(C)V and formation of hilar cholangiocarcinoma are correlated. The patients with hilar cholangiocarcinoma complicated with HB(C)V infection might have higher degree of malignancy in biological characteristics and worse prognosis. The infection of HB(C)V in hilar cholangiocarcinoma can exert significant on p53 protein expression.
出处
《中华肝胆外科杂志》
CAS
CSCD
2003年第3期177-181,共5页
Chinese Journal of Hepatobiliary Surgery
