摘要
目的 探讨选择性一氧化氮合酶 (NOS)抑制剂 7 硝基吲唑 (7 NI)对缺氧缺血新生大鼠海马CA1区神经元凋亡的影响。方法 7日龄Wistar大鼠 72只 ,随机分成 3组 ,(1)假手术组 :12只 ,仅作颈正中切口 ,不作颈总动脉结扎 ;(2 )观察组 (7 NI组 ) :30只 ,缺氧缺血性脑损伤 (HIBD)模型制作后即刻腹腔内注射 7 NI 2 5mg/kg ;(3)对照组 (生理盐水组 ) :30只 ,HIBD后即刻腹腔注射等体积的生理盐水。假手术组于手术后即刻 ,观察组、对照组于处置后 4 8小时断头取脑 ,分别进行HE和TUNEL染色 ,光镜下检测脑细胞凋亡数。结果 HE染色对照组大鼠海马CA1区可见典型的凋亡细胞 ,表现为细胞固缩 ,胞浆深染 ,核浓缩、碎片 ,核浆分布不清 ,有凋亡小体形成 ;7 NI组上述凋亡细胞明显减少 ;TUNEL染色对照组阳性细胞数与 7 NI组比较差异有极显著性 (P <0 .0 1)。结论 7
Objective To explore the effects of neuronal apoptosis in the hippocampal CA 1 region by the selective nitric oxide synthase(NOS) inhibitor, 7 nitroindazole (7 NI).Methods 72 postnatal 7 day old Wistar rats were randomly divided into 3 groups.(1)Sham operation group:12 rats were cut in their neck centre without ligature in common carotid artery;(2)Obersevertion group(7 NI group):the abdominal cavity of 30 rats were injected intraperitoneally into 7 NI (25 mg/kg) after anoxic ischemic damaged models were made;(3)The control group(the normal saline):30 rats were injected intraperitoneally into the normal saline the same volume after HIBD.The sham operative group was later than the operative one.The rats in the observation group and the control one by killed later,they were cut heads and brains in 48h.and were detected by HE and TUNEL stainning,the numbers of cerebral apoptosis were detected under the lightmicroscope.Results Typal apoptotic cells in the hippocampal CA 1 region in the control group were detected by HE stainning. They showed cell pyknosis,cytoplasm deep stain,nucleus concentration,fragment,karyoplasm unclear,apoptosis corpuscle was formed;in 7 NI group the above apoptosis decresed significantly;There was significant difference between Group 2 and Group 3(TUNEL positive apoptotic cells in Group 2)( P < 0.01 ).Conclusion 7 NI has obvious inhibitory effect to the neuronal apoptosis in the hippocampal CA 1 region after HIBD.
出处
《临床神经病学杂志》
CAS
2003年第1期9-11,共3页
Journal of Clinical Neurology
基金
山东省教育厅科技基金资助 (J 99K 0 9)
