摘要
本文研究确定了罗红霉素与甲酚红的荷移反应条件。在乙醇 丙酮介质中 ,罗红霉素与甲酚红在35℃的温度时发生荷移反应 ,产物的最大吸收波长为 456nm ,表观摩尔吸光系数为 1 0 5× 1 0 4L·mol- 1 ·cm- 1 ,罗红霉素的浓度在 0～ 80mg·L- 1 范围内与吸光度呈线性关系 ,相对标准偏差为 0 87% (n=6) ,回收率在 98%以上。测定了荷移络合物的组成比为 1∶1 ,探讨了反应机理 ,其中罗红素作为电子给予体、甲酚红作为电子接受体发生了电荷转移反应。
A spectrophotometric method for the determination of Roxithromycin has been developed based on the charge transfer reaction between Roxithromycin as donor and cresol red as acceptor. The conditions of reaction have been studied. The reaction time, reaction temperature, reaction medium and the concentration of reagent was ten min, 35degreesC, alcohol-acetone solution and 6 X 10(-4) mol.L-1 respectively. The, apparent molar obsorptivity of complex was 1.05 X 10(4) L.mol(-1) . cm(-1) at 456 nm. The Beer's law was obeyed in the range of 0-80 mg . L-1 of Roxithromycin with correlation coefficient 0. 999 7. The recovery was over 98 %, The composition of the charge transfer complex between Roxithromycin and cresol red was 1:1. The reaction mechanism has been discussed based on the composition of the complex. The present method has been applied to the determination of Roxithromycin in capsules with satisfactory results.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2003年第1期157-159,共3页
Spectroscopy and Spectral Analysis
