摘要
目的研究PentaD、PentaE基因座在中国广西、湖南、河南、四川、台湾汉族5个群体遗传多态性,比较它们之间的遗传学差异。方法用ProwerPlex(?)16 System荧光标记试剂盒(Promega公司)检测其多态性。结果PentaD、PentaE基因座在5个群体共646名无关个体中分别检出10、19个等位基因,PentaD在湖南汉族首次检出等位基因19。PentaE在河南汉族首次检出等位基因7。PentaD和PentaE在各群体的期望杂合度分别为0.7746～0.8047和0.9005-0.9219,多态信息含量(PIC)为0.7710～0.8025和0.8969-0.9176,个体识别率为0.9223-0.9341和0.9471-0.9782,非父排除率(PE)为0.5435-0.6325和0.6785-0.8465。结论PentaD、PentaE是高度多态性STR基因座,在法医学中有重要应用价值。
Objective To obtain the genetic polymorphism data of Guangxi, Hunan, Henan, Sichuan ,Taiwang Chinese Han population and compare the polymorphism of PentaD and PentaE STR locus. Methods The two loci was analyzed by using the PowerPlex 16 System. Results 10 alleles of PentaD and 19 alleles of PentaE were found in the five Han population. PentaD and PentaE have the expected heterozygosity values of 0.774 6-0.804 7and 0.900 5-0.921 9,the polymorphism information content values of 0.771 0-0.802 5 and 0.896 9-0.917 6, the discrimination power values of 0.9223-0.934 1 and 0.947 1-0.978 2,the power of exclusion values of 0.5435-0.632 5 and 0.678 5-0.846 5, respectively. Conclusion The result showed that these two loci were highly informative and suitable for forensic application.
出处
《法医学杂志》
CAS
CSCD
2003年第1期24-26,共3页
Journal of Forensic Medicine
