摘要
目的 比较段木栽培灵芝多糖 (wood culturedGanodermalucidumpolysaccharides ,Gl PS WC)及袋栽灵芝多糖 (bag culturedGanodermalucidumpolysaccharides,Gl PS BC)对体外培养小鼠脾淋巴细胞增殖活性的影响 ,探讨袋栽灵芝多糖替代段木栽培灵芝多糖的可能性。方法 检测两种灵芝多糖对混合淋巴细胞培养 (MLC)反应的影响 ;观察对刀豆蛋白A (ConA)、细菌脂多糖 (LPS)诱导淋巴细胞增殖的影响以及对环孢素A (CsA)、丝裂霉素C(MitC)、足叶乙苷 (VP 16)等抑制MLC反应的影响。结果 当质量浓度为 0 2～ 12 8mg·L- 1 时 ,两种灵芝多糖均可促进MLC反应 ,增强ConA或LPS诱导的淋巴细胞增殖 ,并拮抗CsA ,MitC或VP 16对MLC反应的抑制作用。未发现两种多糖之间有显著性差异。结论 Gl PS WC及Gl PS
Aim To compare the influences of wood cultured Ganoderma lucidum polysaccharides ( Gl PS WC) and bag cultured Ganoderma lucidum polysaccharides ( Gl PS BC) on the proliferation activities of murine spleen lymphocytes in vitro , and investigate whether Gl PS BC can be substituted for Gl PS WC. Methods Mixed lymphocyte culture (MLC) reaction, lymphocyte proliferation induced by concanavalin A (Con A, 1 mg·L -1 ) or lipopolysaccharide (LPS, 5 mg·L -1 ), MLC reactions inhibited by immunosuppressive drugs, cyclosporine A (CsA, 0 1 mg·L -1 ), mitomycin (Mit C, 0 1 mg·L -1 ), or antitumor drug, etoposide (VP 16, 0 1 mg·L -1 ), were detected in the presence or absence of Gl PS WC and Gl PS BC in the concentration range of 0 2-12 8 mg·L -1 . Results Two kinds of polysaccharides were shown to promote MLC in the range of 0 2-12 8 mg·L -1 , increase lymphocyte proliferation induced by Con A or LPS and antagonize the inhibitory effects of CsA, Mit C or VP 16 on MLC. No significant difference was observed between these two kinds of polysaccharides in selected concentrations. Conclusion Gl PS WC and Gl PS BC showed similar effects on the proliferation activities of murine spleen lymphocytes in vitro .
出处
《药学学报》
CAS
CSCD
北大核心
2003年第2期92-97,共6页
Acta Pharmaceutica Sinica
基金
ResearchFundofShanghaiGreenValleyHoldingCo .Ltd .
关键词
段木栽培
袋栽灵芝
体外培养
小鼠
脾淋巴细胞增殖活性
比较
灵芝多糖
wood cultured Ganoderma lucidum
bag cultured Ganoderma lucidum
Ganoderma lucidum polysaccharides ( Gl PS)
mixed lymphocyte culture (MLC)
lymphocyte proliferation
murine spleen
