摘要
目的 :探讨神经元特异性烯醇化酶 (NSE)、肿瘤坏死因子 -α(TNF -α)及脂质唾液酸 (LSA)在肺癌中的表达及其诊断价值。方法 :收集肺癌病人血清 78份 ,良性肺病患者血清 32份 ,正常对照血清 10 9份。采用酶联免疫吸附试验 (ELISA)和化学发光免疫分析法分别测定血清中的NSE、TNF -α及LSA。结果 :肺癌患者血清中的NSE、TNF -α、LSA分别为 19 78± 12 10ng/ml、135 6 4± 4 9 0 1pg/ml、1 0 6± 0 31mg/ml,明显高于良性肺病组 7 5 6± 3 4 1ng/ml、84 70± 2 4 89pg/ml、0 78± 0 18mg/ml及正常对照组 8 0 1± 2 81ng/ml、71 2 5± 13 5 0pg/ml、0 70± 0 13mg/ml(p <0 0 1)。对肺癌诊断的阳性率分别为 6 2 82 %、84 6 2 %、75 6 4 。联合检测阳性率达92 31%。结论 :血清NSE、TNF -α、LSA在肺癌病人中有较高的阳性率 ,联检阳性率更高 ,故NSE、TNF -α、LSA的联检是肺癌的非常有用的辅助诊断指标。 阳性标准为NSE >16 0ng/ml,TNF -α >10 0pg/ml,LSA >0 5 8mg/ml。
Objective To explore the expression and the diagnostic value of determining serum neuron-specific enolase(NSE), tumor necrosis factor-α(TNF-α) and lipid-associated sialic acid(LSA) in patients with lung cancer. Methods The concentrations of NSE, TNF-α and LSA were measured in 78 patients with lung cancer and 32 patients with benign lung diseases as well as 109 controls by enzymelinked immunosorbent assay (ELISA) and chemical assay respectively. Results The levels of NSE (19.78±12.10ng/ml), TNF-α(135.64±49 01pg/ml) and LSA (106±0 31ng/ml) were significantly higher in patients with lung cancer than those in patients with benign lung diseases (NSE 7.56±3 41ng/ml, TNF-α 84.70±24.89pg/ml, LSA 0.78±0.18mg/ml) and controls (NSE 8.01±2.81ng/ml, TNF-α 71.25±13 50 pg/ml, LSA 0 70±0 13ng/ml)(all p <0 01).Conclusion The present data suggest that the syntheses of NSE, TNF-α and LSA increase in patients with lung cancer and combined determination of NSE, TNF-α and LSA be helpful to diagnosis of lung cancer. positiveness means: NSE>16.0ng/ml, TNF-α>100pg/ml, LSA>0 58mg/ml.
出处
《放射免疫学杂志》
CAS
2003年第1期63-64,F003,共3页
Journal of Radioimmanology
关键词
血清
NSE
TNF-Α
LSA
联检
肺癌
诊断
neuron-specific enolase
tumor necrosis factor-α
lipid-associated sialic acid
lung cancer
diagnosis
