摘要
目的 探讨 3种流式细胞术计数血小板方法的临床应用价值。方法 用二维激光散射法 (ADVIA 12 0法 )、红细胞与血小板比值法 (RBC/PLT R法 )、荧光微球绝对计数法 (TruCOUNT法 ) 3种流式细胞术 (FCM)方法计数血小板 ,并与电阻抗法比较。结果 3种FCM方法计数血小板具有高度相关性 (P <0 0 0 1) ,血小板计数在 ( 1～ 6 46 )× 10 9/L的较宽范围内时 ,相关系数 (r) >0 99;血小板计数在 ( 1～ 89)× 10 9/L的较低范围内时 ,相关系数 (r) >0 95。与TruCOUNT法血小板计数相比 ,ADVIA12 0法计数值偏高 ,RBC/PLT R法偏低。TruCOUNT法与ADVIA 12 0法的相关性最好 (P <0 0 0 1) ,在较低范围血小板计数时的相关系数 (r) >0 98。低血小板计数时 ,ADVIA 12 0法的变异系数 ( 3 46 % )最小 ,TruCOUNT法 ( 5 2 % )次之 ,RBC/PLT R法 ( 5 84% )稍高。电阻抗法与 3种FCM法均有相关性 (P <0 0 0 1) ,但血小板计数比 3种FCM法偏低 (P <0 0 1) ,而且对部分病例标本的血小板计数存在较大误差。结论 3种FCM法计数血小板均优于电阻抗法。ADVIA 12 0全自动血细胞分析系统最适合于临床常规计数血小板 ,TruCOUNT法是较为低血小板计数和校准血细胞分析仪。对严重血小板减少症的患者 。
Objective To study the clinical application of three kinds of flow cytometric methods for platelet counts. Methods Three kinds of flow cytometric methods for platelet counts by two-dimensional laser scatter method (AdVIA 120 method), RBC/platelet ratio method (RBC/PLT-R method) and absolute counting method by using fluorescent microbeads (TruCOUNT method) and respectively compared them with impedance method. Results Three kinds of flow cytometric methods correlated extremely well ( P<0.001), at platelet in (1～646)×109/L, relative coefficient (r) >0.99. when platelet in(1～89)×109/L, r>0.95. Comparision of TruCOUNT method with ADVIA 120 and RBC/PLT-R method, ADVIA120 was higher and RBC/PLT-R was lower. In low platelet counting, coefficient of variation of ADVIA120 was the smallest (3.46%), followed by TruCOUNT (5.2%), and the highest coefficient of variation was that of RBC/PLT-R (5.84%). Although impedance method and three kinds of flow cytometric methods for platelet counts show a correlation (r>0.98, P<0.001) too, platelet counts by impedance method were lower than that by three kinds of flow cytometric methods, and there was the abnormal error of platelet counting by impedance method in some cases. Conclusion It is proposed that three kinds of flow cytometric methods for platelet counts may be more accurate than impedance method. ADVIA120 automated blood cell analytic system is suitable for clinical routine in platelet counts, it was not only correlative well with immuoplatelet counting method of monoclonal antibody, but also simple and rapid. TruCOUNT method is an ideal method of platelet counting, which is accurate, sensitive and less influenced. So TruCOUNT method is applicable to counting low platelet and calibrating the blood cell analyzer. When the patients with severe thrombocytopenia need prophylactic platelet transfusion, TruCOUNT method can be used for counting platelets accurately and precisely.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2003年第1期8-12,共5页
Chinese Journal of Laboratory Medicine
