摘要
目的研究流式细胞术(FCM)计数血液淋巴细胞亚群的全血质控品。方法流式细胞术多色分析方法计数不同保存剂处理后、不同时间内血液中淋巴细胞亚群。结果成功研制出FCM计数血液淋巴细胞亚群的全血质控品。在2～8℃冰箱内保存72d内,各种白细胞的光散射特点及CD45保持稳定,淋巴细胞、单核细胞和中性粒细胞群与细胞碎片分离良好,可通过光散射或荧光/光散射两种方式设门分析淋巴细胞亚群。本质控品在不同保存时间的变异较小,FCM计数血液淋巴细胞亚群的室内批间变异系数<6.5%,全国56个实验室的室间质评变异系数<13%。结论本全血质控品可用于临床FCM计数血液淋巴细胞亚群全过程的室内质量控制和室间质量评价,对提高淋巴细胞亚群计数的质量,保证结果的准确性有重要意义。
Objective To research on a whole blood control material for lymphocyte subset counting by flow cytometry（FCM）. Methods To detect lymphocyte subset in whole blood with different preservers by flow cytometric multi-color analysis. Results whole blood control material for lymphocyte subset counting by FCM was prepared. In 2-8℃ refrigerator, the light scatter and CD45 of leukocytes of whole blood control were stable in 72 days. The cluster of lymphocyte, monocyte, neutrophil in plot were separated easily from debris. The lymphocyte subset of whole blood control can be counted by FSC/SSC or CD45/SSC gating. The variation of lymphocyte subset count was less in different preserving day. The coefficient of variation （CV） of lymphocyte subset count was less than 6. 5% in our laboratory and less than 13% in external quality assessment among 56 laboratories in China. Conclusion The whole blood control prepared by us can be used for internal quality control and external quality assessment in lymphocyte subset counting by FCM, it is very important signification to ensure the quality and accuracy of lymphocyte subset count.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2005年第9期897-901,共5页
Chinese Journal of Laboratory Medicine
