摘要
目的 :观察LPS对人牙髓成纤维细胞 (HDPF)表达基质金属蛋白酶抑制剂 1,2 (TIMP - 1,2 )的影响。方法 :体外培养HDPF ,用 1、10、10 0 μg/mLLPS分别刺激 2d ,采用免疫组化和图像分析法 ,观察HDPF表达TIMP - 1,2的变化。结果 :不同质量浓度的LPS对TIMP - 1,2的影响不同。与空白组相比 ,10 μg/mL的LPS刺激HDPFTIMP - 1的表达明显增加 (P <0 .0 1) ;LPS质量浓度为 10 0 μg/mL时 ,HDPF表达TIMP - 1明显减弱(P <0 .0 1)。LPS对TIMP - 2的影响呈浓度依赖关系 ;LPS质量浓度≥ 10 μg/mL时 ,TIMP - 2表达显著减弱(P <0 .0 1)。结论 :LPS通过改变牙髓成纤维细胞TIMP - 1,2的表达量 。
AIM:To investigate the influence of LPS on the expression of TIMP-1,TIMP-2 in HDPF.METHODS:TIMP-1?TIMP-2 was stained with immunohistochemical method in HDPF by treatment with different concentration of LPS. Stimulation time is 2 d.RESULTS:There are different expressions of TIMP-1 and TIMP-2 to the stimulation of LPS of different concentration. The TIMP-1 expression was obviously intensified (P<0.01) while HDPF was stimulated by 10 μg/mL LPS.The TIMP-1 expression was obviously weakened (P<0.01) while the concentration of LPS is 100 μg/mL. The influence of LPS on TIMP-2 is concentration related.When the concentration of LPS is ≥10 μg/mL, TIMP-2 expression decreased greatly (P<0.01).CONCLUSION:The results indicate that the LPS affects inflammation by changing the expression of TIMP-1 and TIMP-2.
出处
《牙体牙髓牙周病学杂志》
CAS
2003年第2期59-61,共3页
Chinese Journal of Conservative Dentistry
基金
高等学校骨干教师资助计划资助
