摘要
运用透射电镜对制动应急大鼠肾上腺髓质嗜铬细胞内分泌颗粒的胞吐特性进行研究 ,结果显示 :它具有三种不同的胞吐方式 :单颗粒胞吐、多颗粒连续胞吐及颗粒内容物通过暂时性融合小孔进行胞吐。用电镜细胞立体形态计量法对正常大鼠肾上腺素细胞内两种不同形态颗粒进行测量 ,结果显示 :肾上腺素细胞中致密圆形颗粒的数目较内容浅淡的颗粒多 (P <0 0 1) ;大鼠制动 8h时 ,以致密圆形颗粒的胞吐为主 ,制动 2 4h时 ,则两种颗粒均发生胞吐。以上结果提示 :这两种不同形态的颗粒可能是大鼠肾上腺髓质嗜铬细胞中同一种分泌颗粒的不同发育阶段。
Transmission electron microscopy was used to study the characteristics of exocytosis of chromaffin cells in adrenal medulla during emergency reaction of restrained rats. It was found that three types of exocytosis existed: single granule exocytosis, multigranule successive exocytosis and exocytosis through a temporary fusion pore. Two kinds of granule were estimated by using stereological morphometry. The results showed: the number of dense core granules was more than that of shallow core granules ( P <0 01). When the rats were restrained for 8h, we found that only the dense core granules decreased. When the restrained time was extended to 24h, both two kinds of granule decreased. It is suggested that these two kinds of granules may be the same secretory granules of chromaffin cells, which are in the different developmental stages.
出处
《电子显微学报》
CAS
CSCD
北大核心
2003年第1期7-11,共5页
Journal of Chinese Electron Microscopy Society
