摘要
目的 通过测定胎盘部位子宫静脉血中一氧化氮 (nitricoxide,NO)及内皮素 (endothelin ,ET)的浓度 ,并与外周血中同类物质的对比 ,了解妊高征时胎盘血管的病变程度及其与外周血中血管活性物质浓度改变的关系。方法 分别于剖宫产手术前及手术中抽取胎盘部位子宫静脉血和外周静脉血 ,应用硝酸根还原酶与Griess反应相结合的方法测定NO ;应用放射免疫分析法测定ET。结果 妊高征组胎盘部位子宫静脉血中NO2 -/NO3 -为 (70 2 6± 12 6 0 ) μmol/l,外周血清NO-2 /NO-3 为 (6 5 5 2± 14 88) μmol/l,二者之间无显著差异。妊高征组外周血浆ET水平为 (5 3 72± 15 2 8)ng/L ,胎盘部位子宫静脉血浆ET水平为 (5 2 80± 14 19)ng/L ,两者之间无显著性差异 (P >0 .0 1)。与正常晚孕组比较 ,妊高征组血中ET、NO-2 /NO-3 水平均显著增高 (P <0 .0 1)。结论 妊高征患者的子宫、胎盘循环系统血管舒、缩物质平衡失调 ,血管内皮系统的功能亦遭到破坏 ;妊高征患者胎盘血循环ET、NO水平升高 ,但与外周血中ET、NO水平的升高无关。
Aim To evaluate the damage of endothelial function of placental circulation by measuring and comparing levels of ET and NO in uterine vein draining placenta and peripheral circulation in preeclampsia. Methods: 25 preeclamptic women were enrolled in this study. Peripheral venous blood samples were collected from all the cases at the beginning of Cesarean section and uterine vein blood samples were taken from the uterine vein draining the placenta before uteruses were incised. Serum NO - 2/NO - 3 the end products of NO, were measured with Gricess reaction with nitrate reduclase. Plasma ET were measured by radioimmunoassay. Results: The mean NO - 2/NO - 3 in peripheral circulation and uterine?placenta circulation were (65.52±14.88)μmol/l and (70.26±12.60)μmol/l respectively in preeclampsia. The mean endothelin in peripheral circulation were (53.72±15.28)ng/L, and (52.80±14.19)ng/L in placental circulation in peeclamptic group. Statistic differences of ET or NO between placental circulation and systemic circilation were not found in two groups while patients with preeclampsia had higher concentrations of ET and NO compared with those of normotensive patients (P<0.01). Conclusion: The function of vascular system of placental is also damaged, which can be demonstrated by the increased ET and NO in placental circulation.
出处
《中国优生与遗传杂志》
2003年第1期91-92,95,共3页
Chinese Journal of Birth Health & Heredity
关键词
妊高征
胎盘
一氧化氮
内皮素
Preeclampsa
Placenta
Endothelin
Nitric Oxide.
