摘要
观察脂多糖 (LPS)介导的大鼠发生急性肺损伤 (ALI)时血浆中IL 13含量的变化 ,探讨ALI发生、发展过程中 ,抗炎因子变化的意义。由Wistar大鼠尾静脉注射梯级剂量LPS(2mg/kg、4mg/kg、6mg/kg、8mg/kg) ,复制ALI模型 ;采用酶联免疫吸附试验 (ELISA)检测血浆IL 13含量。结果发现 ,梯级剂量LPS可以引发大鼠程度不同的ALI,当LPS≥ 6mg/kg时 ,发生急性呼吸窘迫综合征 (ARDS) ;LPS可以导致大鼠血浆IL 13含量升高 ,当LPS≥ 6mg/kg时 ,血浆IL 13含量峰值显著提高。研究表明 ,尾静脉注射LPS可以复制大鼠ALI模型 ,LPS≥ 6mg/kg是大鼠发生ARDS的临界剂量 ;LPS所介导的大鼠血浆IL 13含量升高 。
To investigate the interleukin 13(IL 13) content in plasma in rats with acute lung injury (ALI) induced by lipopolysaccharide (LPS), Wistar rats were given increasing doses of LPS (2mg/kg,4mg/kg,6mg/kg,8mg/kg) to produce ALI. The respirtory rate,PaO 2 ,wet weight/dry weight (W/D) of the lung, and pathological changes in the lung were observed. ELISA was used to determine plasma IL 13. It was found that: (1)ALI could be produced in rats with LPS, but ARDS occurred only when the dose of LPS reached 6mg/kg. or larger. (2)LPS produced an elevation of the content of IL 13 in plasma in rats, peaking when the dose of LPS reaching 6mg/kg or over. These results suggested that LPS might induce ALI in rats, and ARDS could be produced when the dose of LPS reached ≥6mg/kg. (3)The high increase in plasma IL 13 content might play an important role in producing ARDS induced by LPS.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2003年第2期107-109,共3页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金重点项目 (编号 39730 2 1 0 )
全军医学科研"十五"重点项目 (编号 0 1Z0 74)资助课题
