摘要
试验者为小细胞肺癌患者,于右上臂皮下植入缓释VP 16(又称依托泊苷或鬼臼乙叉苷),按预定时间点采集静脉血并分离出血浆。血浆样品以氯仿萃取,常温下负压挥发至干,然后以流动相溶解,采用反相高效液相色谱法测定。流动相为V(甲醇)∶V(水)=52∶48的溶液,检测波长为UV 228nm,色谱柱为LichrosphereC18(250mm×4 6mmi d ,5μm)。用外标法定量。实验结果表明,该测定方法的检出限为0 02mg/L,绝对回收率为80 4%,相对回收率为94 7%,日内和日间精密度(用相对标准偏差RSD表示)均为8%左右;VP 16与血浆中其他成分(内源性干扰物)达到良好分离。此外还进行了血浆样品的稳定性试验,发现VP 16的萃后残渣于-5～0℃可保存240h。方法灵敏度较高,准确性和实用性好,为临床应用提供了可靠的检测方法。
The patients with cellule lung cancer were implanted sustained releaseetoposide (VP16) on the right upper arms. The plasma was separated from the vein blood collected at prearranged times from these patents. The plasma sample was extracted with chloroform, and evaporated at ordinary temperature under reduced pressure. Then it was dissolved by the mobile phase, and determined by external standard reversedphase high performance liquid chromatography with a Lichrosphere C18 (250 mm×46 mm id,5 μm) using methanolwater (10∶90 in volume ratio) as the mobile phase at the detection wavelength of UV228 nm. The detection limit was 002 mg/L. The absolute recovery was 804%. The relative recovery was 947%. The RSDs of intraday and interday were about 8%. With the method VP16 was well separated from the other components in the plasma. The sample could be preserved as residue in dry state after pretreatment at -50 ℃ for 240 h . This method is reliable, accurate and sensitive, and can be used in practical clinics.
出处
《色谱》
CAS
CSCD
北大核心
2003年第1期56-59,共4页
Chinese Journal of Chromatography
基金
中华人民共和国科学技术部基础研究快速反应支持项目(国科基字:[2001]51号).
关键词
反相高效液相色谱法
缓释VP-16
血药浓度
reversed-phase high performance liquid chromatography
etoposide sustained release
plasma drug concentration
