摘要
目的 :考察自体骨膜吸附包裹多孔双相磷酸钙生物活性陶瓷肌内植入的生物力学和成骨活性 ,为骨缺损修复替代材料的选择和制备提供实验依据。方法 :用自体骨膜包裹多孔双相磷酸钙生物活性陶瓷植入 2 1只狗的肌内 ,与未包裹骨膜的同样材料肌内植入和骨内植入作对照 ,进行生物力学测定、X线衍射和红外光谱分析以及组织学观察。结果 :发现实验组材料的抗弯强度随着时间的延长而增加 ,其程度虽不如骨内植入者 ,但远高于未包裹骨膜的肌内植入试样 ,3月和 6月时已接近或达到正常骨水平 ;X线衍射结果 ,实验组TCP的峰值下降明显快于未包裹骨膜的肌内植入组 ,6月时X线转靶谱图和红外光谱图已接近正常骨的谱图。组织学观察也证实实验组试样的成骨速度和质量显著高于未包裹骨膜的肌内植入试样。结论 :活体骨膜可为多孔生物活性陶瓷材料提供丰富的生长因子 ,激活和提高材料的成骨活性 ,有利于骨诱导和新骨形成 ,促进骨的修复替代。
Objective:To observe the biomechanical and osteogenic properties of implanted composite made from autogenic periosteum and biphase porous active bioceramic.Methods:The biphase porous hydroxyapatite tricalcium phosphate(HA-TCP)ceramics wrapped by autogeneic fresh periosteum were implanted into the anterior femur muscle(experimental group)of 21 dogs.The control was established by implantation of the same HA-TCP ceramics without autogeneic periosteum into the bone(control group A)and into the posterior femur muscle(control group B).Biomechanical test was employed to assess the bending strength and histological study was used to observe the osteogenic speed and quality.The rate and speed of TCP were detected by X-ray diffraction and infrared spectrum analysis.Results:The biomechanical test revealed a significant higher bending strength in experimental group when compared with thaat in control group B,which almost reached the degree of the normal bones six months after operation.The results of X-ray diffraction and infrared spectrum analysis showed that the falling speed of TCP peak value in experimental group was faster than that in control group B,but slower than that in control group A.A higher osteogenic speed and better osteogenic quality were observed by histologic study.Conclusion:Fresh autogeneic periosteum can provide abundant growth factors for the porous active biocaramic to activate and increase its osteogenic properties,which can improve the osteoinduction and osteogenesis.
出处
《西南国防医药》
CAS
2003年第1期14-17,共4页
Medical Journal of National Defending Forces in Southwest China
关键词
磷酸钙基陶瓷
骨膜
生物活性
骨诱导
calcium phosphate ceramic
periosteum
bioactivity
osteoinduction
