摘要
小麦黄花叶病毒(Wheat yellow mosaic virus,WYMV),属于马铃薯Y病毒科(Potyviridae),大麦黄花叶病毒属(Bymovirus),传播介体为禾谷多粘菌(Polymyxa graminis),与发生在欧美的小麦梭条花叶病毒(WSSMV)为同一属内的两种病毒[1].
With purified Wheat yellow mosaic virus (WYMV) particles as antigen, cavy was injected to produce specific antiserum. Its titer was tested as 1/128 by the method of micro-immunoprecipitation. Using cavy antiserum as capture antibody for trapping the virus and rabbit antiserum as detection antibody, the experiment system of heterogeneous animal DAS-ELISA (HADAS-ELISA) was established to detect WYMV. The system has the advantage of better specificity and sensitivity of detection. This method was proved to be successful and prattical in the detection of the virus in field-collected samples , indicating that the experiment system of HADAS-ELTSA is superior to the indirect ELISA with rabbit antiserum only.
出处
《中国病毒学》
CSCD
2003年第1期76-78,共3页
Virologica Sinica
基金
国家自然科学基金资助(39870489)
"973"项目资助(G2000016201)
