摘要
本研究针对来自江苏、河南、四川、湖北、日本和美国的37个小麦品种,在进行小麦抗黄花叶病的抗病性鉴定、调查的基础上,结合分子生物学检测(RT-PCR和ELISA),证实小麦品种扬辐9311对小麦黄花叶病毒(WYMV)表现免疫。将该品种与表现高感的小麦品种进行杂交和回交,通过对其后代抗感分离的调查与分析,确定了小麦品种扬辐9311对黄花叶病的抗性受一对显性基因控制,并为寻找小麦抗黄花叶病基因的分子标记提供理论依据和实验材料。同时,本文对小麦抗黄花叶病表现的影响因素及宁麦9号的抗病性进行了分析和讨论。
Wheat yellow mosaic disease resistance was identified in the field among thirty-seven wheat varieties derived from China (Jiangsu, Henan, Sichuan and Hubei Province), Japan and United States. By RT-PCR and ELISA detections, it was proved that the wheat variety 'Yangfu 9311' displayed immunity to Wheat yellow mosaic virus(WYMV). The variety was crossed and backcrossed with those that were susceptible to the disease. By screening for the resistance among the segregating populations of F2 and BC1 the inheritance studies indicated that the resistance of 'Yangfu 9311' was controlled by a pair of dominant monogene. These results provided theoretical basis and experimental materials to find molecular markers of the resistance gene. In this paper, the resistant phenotype of Ningmai 9 and the influencing factors for resistance to Wheat yellow mosaic disease were also discussed.
出处
《植物病理学报》
CAS
CSCD
北大核心
2004年第6期542-547,共6页
Acta Phytopathologica Sinica
基金
国家自然科学基金项目(30270874
39870489)资助
