摘要
目的 探讨一氧化氮 (NO)在膀胱肿瘤中的作用及与肿瘤血管形成的关系。 方法 采用免疫组化方法对 5 8例膀胱移行细胞癌及 14例正常膀胱粘膜标本进行一氧化氮合酶 (NOS)抗体染色和微血管密度 (MVD)计数 ,观察表达结果与肿瘤生物学特性及与肿瘤血管形成的相关性。 结果 诱导型NOS(iNOS)在正常膀胱组织中无表达 ,而在膀胱癌组织中的阳性表达率为 81% ( 47/5 8) ,表达程度与肿瘤分级、分期无关 (P >0 .0 5 ) ;iNOS表达阳性组和阴性组的MVD分别为 ( 3 9.3± 19.5 )和( 2 9.3± 10 .5 ) /HP ,即iNOS的表达程度与肿瘤MVD成正比 (P <0 .0 1) ;内皮型NOS(eNOS)在膀胱肿瘤和正常膀胱粘膜的血管内皮细胞都有明显表达 ,而移行上皮细胞中未见表达。
Objective Evaluated NOS expression in bladder tissue from the patients with transitional cell carcinoma (TCC) of the bladder and studied its relationship with angiogenesis. Methods Bladder carcinoma tissue specimens were procured from 58 patients with TCC and 14 cases of benign bladder tissue as contrast group. NOS immunohistochemistry was performed on all tissue specimens and microvessal density (MVD) was counted by endothelial cells immunostained. Results Inducible NOS specific proteins were found in 47 of 58 bladder cancer specimens but not in control bladder tissue. The malignant cells and inflammatory cells within the carcinomas were highly iNOS positive whereas specimens of bladder mucosa outside the malignant regions showed only a weak positive iNOS immunostaining. The endothelial cells in both normal urothlium and tumor tissue showed a highly positive eNOS immunostaining but its immunoreaction was not detected in either malignant or benign epithelium. MVD was (39.3±19.5)/HP and (29.3±10.5)/HP in iNOS positive and negative tissues respectively (P< 0.01). A correlation between iNOS immunoreactivity and tumor grade in bladder carcinoma could not be verified. Conclusions Bladder carcinoma tissue had a high iNOS content,which plays an important role in tumor angiogenesis.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2003年第2期99-101,共3页
Chinese Journal of Urology
基金
福建省卫生厅青年基金 (闽卫教 2 0 0 0 35 5号)
关键词
膀胱肿瘤
一氧化氮合酶
肿瘤血管组织
Bladder neoplasms
Carcinoma
Nitric oxide synthase
Neoplasms,vascular tissue
