摘要
目的 :了解当归补血汤及大黄栀子组方对创伤早期大鼠外周血象的影响 ,探讨两药方对创伤早期的应激调理作用。方法 :大鼠随机分为当归补血汤治疗组、大黄栀子组方治疗组、模型对照组和正常对照组。两个治疗组及模型对照组采用落体砸击方式造成鼠双下肢闭合性骨折创伤应激模型 ;伤后开始分别给各组实施胃灌注中药或生理盐水 ,正常对照组则仅灌胃生理盐水 ,每日 2次 ;于伤后 4 h、12 h和 2 4 h分别抽血进行血常规检查。结果 :伤后 4 h,外周血白细胞总数及中性粒细胞均明显升高 ,血小板则明显降低 ;12 h后白细胞总数有所下降 ;2 4 h后白细胞总数复又有所上升。两个中药方组均可控制白细胞的升高 ,防止血小板的下降 ,以伤后4 h的作用为著 (P均 <0 .0 1) ;大黄、栀子组方组对白细胞的控制时间长于当归补血汤组。结论 :中药当归、黄芪及大黄等组方能有效控制创伤应激时炎细胞的升高 ,并减少血小板消耗 ,其作用高峰与应激素释放时相相符 ,两个组方通过不同作用机制具有创伤应激调理的功效。
Objective:To evaluate preventing rules of Danggui Buxue decoction(当归补血汤) and prescri ption containing rheum officinale bail(大黄栀子组方) on trauma stress.Methods:Forty Wistar rats were randomly divided into Danggui Buxue decoction treatment group (therapyⅠ),prescription containing rheum treatment group(therapyⅡ),trauma control group and health control groups.The bone trauma animal model were made by smashing of 1 kilogram weight iron fall from 50 centimetre height and were treated two times daily with Danggui Buxue decoction,prescription containing rheum officinale bail and gardenia jasminoides ellis respectively by oral at 1 hour after injured.Trauma and health control rats were given 0 9% NaCl respectively at the same time.The peripheral blood were collected and mononuclears and platelet in it were analyzed by conventionality test.Results:The number of total white blood cells (TWBC) and neutrophil increased significantly while platelet decreased at 4 hours after injured.The TWBC began to restore at 12 hours but increased again at 24 hours after injured.The number of TWBC in therapy Ⅰ and therapy Ⅱ groups lower while platelet higher than that of control ( P <0 01),especially at 4 hours after injured.The preventing rule in therapy Ⅱ group longer than that of therapy Ⅰ.Conclusion:Danggui Buxue decoction and prescription containing rheum officinale bail and gardenia jasminoides ellis have the ability to control inflammatory cells increasing and platelet consuming in peripheral blood.The peak effective time consistent with the stress hormone secreting in the same kind of trauma animals.The results indicate that those two herb prescriptions can regulate stress reaction by different mechanism.
出处
《中国中西医结合急救杂志》
CAS
2003年第1期34-36,共3页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
成都军区科研基金资助项目 (99A0 0 4)
