摘要
本文对硫鸟嘌呤在不同的固体基质和重原子微扰剂存在下的RTP发射强度进行了比较。结果表明,适宜的固体基质是国产慢速定量滤纸,有效重原子盐为NaI或In_2(SO_4)_3。在此基础上对响影硫鸟嘌呤RTP发射强度的各种因素进行了研究,建立了测定痕量硫鸟嘌呤的SS-RTP法。以NaI为重原子时,方法的线性范围为3.3～200.4ng,检出限为0.4ng/斑点。以In_2(SO_4)_3为重原子时,线性范围为3.3～334.3ng,检出限为1.6ng/斑点。
Room temperature phosphorescence of thioguanine was induced on filter paper in the presence of NaI and In_2(SO_4)_3 acted as effective heavy atom perturbers, Different heavy atom effects of NaI and In_2(SO_4)_3 were compared. Experimental conditions of heavy atom using NaI and In_2(SO_4)_3 were studied in detail. Analytical calibration curve of thioguanine are linear in 3.3～200.4ng for NaI, 3.3～334.3ng for In_2(SO_4)_3 The limit of the determinations is 0.4ng/spot for NaI, 1.6ng/spot for In_2(SO_4)_3.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1992年第10期1163-1166,共4页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金
山西省自然科学基金资助课题
关键词
滤纸基质室温燐光法
硫鸟嘌呤
Paper substrate room temperature phosphorimetry
Thioguanine
