摘要
本文研究了高灵敏的meso-四(4-乙酰氧基苯)卟啉[T(4-AOP)P]-铜(Ⅱ-乳化剂OP体系。在酸度为pH5.0～5.6范围内形成的三元络合物可引起T(4-AOP)P-OP荧光强度的定量熄灭,其Ex=413nm,EM=656.9nm,Cu(Ⅱ)浓度在0～0.150μg/ml范围内有良好的线性关系,回归方程F=25.51-8.45m,试验了14种离子的干扰影响,并用此法测定了小球藻中Cu的含量,回收率达96～104％。
In the presence of surfactant octyl phenyl pclyglycol ether (OP), Cu(Ⅱ) reacts with meso-tetracetylphenylporphyrin [T(4-AOP)P] in aqueous solution at pH 5.0～5.6, and results in fluorescence quenching of T(4-AOP)P-OP. This effect can, be used for the fluorescence determination of Cu(Ⅱ). The fluorescence is measured at 656.9nm. The calibration curve is linear for Cu(Ⅱ) from O to 150 ppb. This method has been used to determine Cu (Ⅱ)in alsa, and the results are satisfactoryily agreed with those obtained by atomic absorp tion spectrophotometry.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1992年第8期918-920,共3页
Chinese Journal of Analytical Chemistry
关键词
铜
卟啉
荧光熄灭法
小球藻
meso-Tetracetylphenylporphyrin
Copper
Fluorescence quenching method.
