摘要
目的 :观察植物雌激素木黄酮和雌激素对培养的人垂体催乳素瘤细胞增殖和凋亡的影响 .方法 :将木黄酮(genistein ,GST)、β 雌二醇 (β E2 )作用于体外培养的人催乳素瘤细胞 ,测定MTT值及3 H TdR掺入量 ,流式细胞仪测定细胞周期 ,并用TUNEL法观察细胞凋亡情况 .结果 :GST可抑制催乳素瘤细胞的增殖 ,并存在着剂量效应 ,1× 10 -5mol·L-1GST可使G1期的细胞比例从对照组的 0 .5 5上升为0 .90 .β E2 以剂量依赖方式刺激催乳素瘤细胞的增殖 ,并使G2期的细胞比例从对照组的 0 .16上升为 0 .4 2 .不同浓度的GST均明显促进催乳素瘤细胞的凋亡 ,E2 对GST的促凋亡作用无明显影响 .雌激素受体阻断剂可阻断雌激素的作用 ,但对木黄酮的作用无影响 .结论 :木黄酮对培养催乳素瘤细胞的影响与雌激素的作用不同 ,木黄酮在体外能明显抑制培养人催乳素瘤细胞的增殖 ,促进其凋亡 ,并不受雌激素受体阻断剂的影响 .
AIM: To study the influence of genistein (GST) and Estrogens on the proliferation and apoptosis of cultured human prolactinoma cells. METHODS: MTT method and 3H TdR incorporation and cell cycle analysis were used to examine the changes in the proliferation and DNA synthesis of human prolactinoma cells under influence of GST and β estradiol (β E 2). Tdt mediated dUTP nick end labeling (TUNEL) test was employed to observe the effect of GST and E 2 on the apoptosis of human prolactinoma cells. RESULTS: In a dose dependent manner, GST could significantly inhibit the proliferation of human prolactinoma cells cultured in vitro . GST (1×10 -5 mol·L -1 ) could increase the proportion of cells in G1 phase from 0.55 up to 0.90. E 2 could dose dependently increase the proliferation of human prolactinoma cells, and the proportion of cells in G2 phase from 0.16 up to 0.42. GST in different concentrations, rather than E 2, could significantly induce the apoptosis of human prolactinoma cells cultured in vitro . Tamoxifen could inhibit the effects of E 2 without any influence on the effects of GST. CONCLUSION: The effect of GST differs from that of E 2 on the human prolactinoma cells in vitro . GST inhibits the proliferation and DNA synthesis of the cultured human pituitary tumors cells, and induces cell apoptosis, which is not blocked by tamoxifen.
出处
《第四军医大学学报》
北大核心
2003年第1期14-16,共3页
Journal of the Fourth Military Medical University
基金
国家自然科学基金资助项目 (30 1 70 353)
