摘要
目的 :观察模拟失重大鼠脑与后肢动脉血管组织血管紧张素Ⅱ 1型受体 (AT1)在基因与蛋白水平的表达是否发生差别性改变 .方法 :以 4wk尾部悬吊大鼠模型模拟失重对动脉血管的影响 .用逆转录 多聚酶链式反应 (RT PCR)检测模拟失重 (SUS)组与同步对照 (CON)组大鼠基底动脉和股动脉血管组织AT1的两种亚型 ,即AT1a和AT1b的mRNA表达水平 ;用Western印迹分析测定两种动脉组织AT1的蛋白表达水平 .结果 :SUS组大鼠基底动脉组织AT1a与AT1b的mRNA及AT1的蛋白表达水平均显著高于CON组 (P <0 0 5 ) .在股动脉组织的改变恰相反 :SUS组AT1a的mRNA表达显著低于CON组 (P <0 0 5 ) ,AT1b的表达无显著改变 ;SUS组AT1的蛋白表达也显著低于CON组 (P <0 0 5 ) .结论 :模拟失重可分别引起大鼠脑动脉与后肢动脉血管组织血管紧张素Ⅱ 1型受体的基因和蛋白表达发生增高和降低的差别性改变 .以上发现进一步支持局部肾素
AIM: To determine whether gene and protein expressions of angiotensin Ⅱ type 1 receptor (AT1)in different rat arterial t issues are differentially regulated under the conditions of simulated weightless ness. METHODS: Male SD rats were randomly assigned to e ither tail-suspended group (SUS) or control group (CON). Reverse transcription-p olymerase chain reaction (RT-PCR) was used to examine the mRNA expressions of tw o subtypes of AT1 (AT1a and AT1b). Western blotting was used to measure the prot ein expression of AT1. RESULTS: In basilar arterial tis sues, the mRNA expressions of both AT1a and AT1b and the protein expression of A T1 were significantly increased in SUS group as compared with those of CON (P <0 05). In femoral arterial tissues, the expression of AT 1a mRNA was significantly decreased in SUS group as compared with that of CON ( P <0 05), but AT1b mRNA expression didn't show any sign ificant changes. The protein expression of AT1 was significantly decreased in SU S group (P <0 05). CONCLUSION: The gene and protein expressions of angiotensinⅡ type 1 receptor seem to be up-and down-regulated in cerebral and hindquarter arterial tissues, respectivel y. These findings support the hypothesis that local rennin-angiotensin system ma y play a pivotal role in the differential adaptation of vessels to microgravity.
出处
《第四军医大学学报》
北大核心
2003年第2期160-164,共5页
Journal of the Fourth Military Medical University
基金
国家自然科学基金 (No .30 1 71 0 32 )
全军医药卫生科研基金 (0 1MB1 2 2 )资助项目
关键词
模拟失重
大鼠
动脉
血管紧张Ⅱ1型受体
weightlessness simulation
rats
art eries
renin angiotensin system
receptors angiotension
reverse transcriptase- polymerase chain reaction
blotting,Western
