摘要
目的 探讨 p2 7KIP1和CyclinE基因蛋白与喉鳞状细胞癌 (LSCC)生物学行为的关系。 方法 采用免疫组化法检测 6 0例LSCC、4 0例喉不典型增生病变和 2 0例喉正常粘膜中 p2 7KIP1和CyclinE基因蛋白的表达。结果 p2 7KIP1和CyclinE阳性表达主要定位于细胞核 ;在LSCC、喉不典型增生病变和喉正常粘膜中 p2 7KIP1阳性表达率分别为 :5 8.3% (35 / 6 0 )、6 7.5 % (2 7/ 4 0 )和 90 .0 % (18/ 2 0 ) (P <0 .0 5 ) ;CyclinE阳性表达率分别为 :4 5 .0 % (2 7/ 6 0 ) ,2 0 .0 % (8/ 4 0 ) ,5 .0 % (1/ 2 0 ) (P <0 .0 0 1)。LSCC中颈淋巴结转移组和非淋巴结转移组 ,淋巴结转移阳性组 p2 7KIP1阳性表达率明显低于阴性组 (P <0 .0 1) ;淋巴结转移组CyclinE阳性表达率明显高于非淋巴结转移组 (P <0 .0 1)。p2 7KIP1与CyclinE表达显著相关性 (P <0 .0 1)。 结论 p2 7KIP1和CyclinE异常表达可能是喉癌发生中早期分子事件 。
Objective To clarify whether immunohistoche mi cal detection of p27 KIP1 and CyclinE might provide prognosis information,w e investigated the expression of p27 KIP1 and CyclinE in laryngeal squamous cell carcinoma (LSCC).Methods The expression of p27 KIP1 and CyclinE was examine d by immunohistochenistry method in 20 cases of nomal epithlia,40 cases of dyspl astic epithlia and 60 cases of LSCC.Results Immu-noreaction to p27 KIP1 and CyclinE were local ized in the nuclei of cells.In nomal and dysplastic squamous epithlium,and in sq uamous cell carcinoma of the larynx,the positive immunostaining rate of p27 K IP1 protein was 90.0%(18/20),67.5%(27/40) and 58.3%(35/60) respectively(P<0.05);the positive immunostaining rate of cyclinE protein was 5 .0%(1/20),20.0%(8/40) and 45.0%(27/60)(P<0.001).p27 KIP1 expression was observed more frequently in the tumors with no lymph node metasta sis;CyclinE expression was observed more frequently in the tumors with lymph nod e metastasis (P<0.01).The positive expression of CyclinE closa ly related with the positive expression of p27 KIP1 (P<0.0 1).Conclusion p27 KIP1 and CylinE could be early molecular ev ents in the carcinogenesis of larynx.Expression of them may contribute to the pr ogression of laryngeal tumors.
出处
《济宁医学院学报》
2002年第4期17-20,共4页
Journal of Jining Medical University
