摘要
目的探讨以间充质干细胞(MSC)与同种异体脱钙骨复合培养法构建组织工程化生物衍生骨的可行性。方法预先制备同种异体脱钙骨,取健康成人骨髓,用单核细胞分离液分离MSC,间充质干细胞基础培养基原代和传代培养。倒置显微镜下观察细胞生物学特性,用FITC标记的抗CD105对第3代细胞进行流式细胞鉴定,并与同种异体脱钙骨复合培养1周后行扫描电镜观察。结果原代及传代培养的MSC增殖迅速,第3代CD105阳性细胞占64.1%,复合同种异体脱钙骨培养1周后细胞生长状态良好,增殖迅速,在材料表面形成细胞层。结论未诱导的MSC是骨组织工程适宜的种子细胞,与同种异体脱钙骨复合可作为骨组织工程的载体。
Objective To investigate the feasibility of using decalcified bone matrix loaded with mesenchymal stem cells (MSCs) derived from adult human bone marrow as scaffolds for bone tissue engineering. Methods Allogenic decalcified bone matrix was prepared. MSCs were isolated from adult human bone marrow with monocyte-separating medium and then cultured in basic medium of mesenchymal stem cells (MSC). The third generation of the cultured cells were identified by FITC-antiCD105, seeded onto allogenic decalcified bone matrix followed by observation under scanning electron microscope and invert microscope a week later. Results Rapid proliferation of both primary and subcultured MSCs was observed, and CD105-positive cells reached 64.1% among the third generation of the cells. MSCs also grew well and proliferated rapidly after being cultured with decalcified bone matrix. Conclusion Naive MSCs are ideal seeding cells for bone tissue engineering, and the allogenic decalcified bone matrix loaded with MSCs may be used as a good scaffold.
出处
《第一军医大学学报》
CSCD
北大核心
2003年第1期40-42,共3页
Journal of First Military Medical University
基金
广东省攻关课题(2001A302020401)~
