摘要
目的:探讨白血病抑制因子(LIF)在子宫内膜异位症(EM)导致不孕机制中的作用。方法:采用原位杂交及免疫组织化学方法对15例异位症并发不孕患者黄体中期子宫内膜中的LIF mRNA及其蛋白的表达进行定位及半定量分析,并以15例有生育妇女作为对照组。结果:子宫内膜异位症组LIF mRNA平均光密度为3.12±0.32,LIF蛋白平均光密度为9.31± 2.10,显著低于对照组的6.25±2.14与15.70±1.52(P<0.05)。结论:子宫内膜异位症组黄体中期子宫内膜LIF表达下降可能是其不孕的原因之一。
Objective: To evaluate the possible role of leukemia inhibitory factor (LIF) in infertile women with endometriosis. Methods: By immunohistochemical analysis and in situ hybridization,the LIF mRNA and protein expressions were determined and semi-quantitatively analyzed in luteal middle phase endometrium of infertile women with endometriosis. Data was compared with 15 normal fertile women without endometriosis in control group. Results: Mean optical density of LIF mRNA and LIF protein was significant lower in endometriosis group than in the control group. Conclusion: Expression of LIF in luteal middle phase endometrium of infertile women with endometriosis is reduced and it may be a possible cause of infertility with endometriosis.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2002年第6期355-357,T002,共4页
Reproduction and Contraception
