摘要
在植物透射电镜制样过程中,由于样品表面结构及化学组成等方面的差异,使按常规程序包埋的样品与包埋剂脱离而不能进入切片以后的各程序。我们在植物叶片电镜制样过程中也遇到了这一问题。经过摸索,找到了一种切实可行且不影响观察效果的简易补救方法。现介绍如下,以供参考。一、样品材料原固定包埋的为植物叶片(人参—Panax Ginseng)。其结构特点是表皮毛较少,叶表皮的蜡质较多,固定的电镜样品很容易在表皮和包埋剂之间分离而脱落。
This paper presents a remedial method for the loose plant tissue in the embeded electron microscopic cube by means of reembedding. The loose tissue was softly treated with acetone and new embedding agent to increase the permeating of the agent into the tissue. The balance period at low temperature(30℃)was prolonged before the embedded cube was polymerized. With the above treatments the agent can perfectly permeated into the tis-sue. The newly embedded tissue can be trimmed, sliced well. The sections showed good resolution under elec-tron microscope.
出处
《电子显微学报》
CAS
CSCD
1992年第3期222-223,共2页
Journal of Chinese Electron Microscopy Society
