摘要
铁蛋白不仅存在于正常人肝、脾及骨髓的单核巨噬系统,同时也在粘膜及血细胞等组织中发现。正常情况下,血液有形成分中的铁蛋白很少,但在某些血液病及肿瘤发生时可出现不同程度的铁蛋白增高。因此,可以通过铁蛋白的变化,对某些疾病的早期诊断提供有价值的线索。我们用抗人铁蛋白抗体结合胶体金免疫技术对正常人外周血细胞进行铁蛋白定位观察。取静脉血2—4ml,1000—1500r/min离心10分钟,戊二醛固定后按常规制作电镜标本。
The uhrastructural localization of ferritin was studied by protein A-gold method in normal peripheral blood
cells. After ferritin immunogold staining for neutrophils, the positive colloidal gold granules were always localized in
the granules of the neutrophils. Although there was little ferritin measured in blood components from normal sub-
jects, ferritin increased in patients with leulcaemia and tumour. It was suggested that the ferritin in peripheral
blood cells could be localized uttrastructurally by protein A-gold method Which was helpful for the eally diagnosis of
some diseases.
出处
《电子显微学报》
CAS
CSCD
1992年第2期157-158,共2页
Journal of Chinese Electron Microscopy Society
