摘要
目的：研究骨髓基质细胞支持造血的分子基础。方法；应用免疫胶体金单标和双标记技术，在长期培养的骨髓基质细胞（HLTBMC）粘附层中，对各种造血调节有关的粘附蛋白进行了免疫电镜定位。结果：长期培养的骨髓基质细胞上均可显示各种粘附蛋白的表达，一些双标记证明各种粘附蛋白与某些细胞的特异性标记（vWF和Fb-sp）是共存的，表明在HLTBMC中的内皮细胞和成纤维细胞可合成和分泌各种粘附蛋白，这些粘附蛋白结合在基质细胞表面膜和细胞外基质上，结论：粘附蛋白与基质细胞分泌的各种生长因子一起，构成了一个支持造血的复杂网络，是基质细胞支持造血的分子基础。
Objective: To study the molecular basis of bone marrow stromal cell supporting hematopoiesis. Methods:Immunoelectron microscopic localization of adherent protein related to regulation of hematopoiesis was performed by immunocolloidal gold labelling technique in the adherent layer of long term cultured human bone marrow stromal cells (LTCHBMSC). Results: Adherent proteins were found to be expressed on LTCHBMSC. Double labelling revealed co-expression of these proteins and some spe cif ic markers for endothelial cells (vWF) and fibroblasts (Fb-sp). It was suggested that endothelial cells and fibroblasts in LTCHBMSC could synthesize and secrete adherent proteins,which bound to the surface of stromal cells and extracellular matrix. Conclusion: Adherent proteins and all kinds of hematopoietic growth factors secreted by stromal cells constitute a complex network supporting hematopoiesis.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1997年第9期468-471,共4页
Chinese Journal of Hematology
基金
国家自然科学基金!39370366
关键词
细胞培养
骨髓基质细胞
粘附蛋白
Cell culture Bone marrow stromal cell Adherent molecule immunocolloidal gold labelling
