摘要
给去势公山羊瘤胃灌注β-酪啡肽(β-CM),在灌注前后采取外周血样,用放射免疫分析法测定血清促卵泡素(FSH)、促黄体素(LH),旨在研究β-酪啡肽对去势公山羊生殖内分泌的影响。结果表明,瘤胃灌注β-CM后,公山羊血清LH水平明显降低(P<0.01),且此效应可被阿片受体拮抗剂纳络酮(naloxone)逆转,而β-CM灌注,对公山羊血清FSH水平未见明显影响(P>0.05)。
15 castrated male goats were divided into 3 groups at random,and ruminally infused with βcasomorphin (βCM),naloxone (Nal)+βCM and 5% NaHCO2 (as control).Blood samples were collected from jugular veins before and after infusion.The serum levels of follicular stimulating hormone(FSH)and luteinizing hormone (LH) were measured by radioimmunoassay(RIA) to determine the effect of βcasomorphin on reproductive endocrine of castrated male goats.The results indicated that ruminal infusions of βCasomorphin significantly decreased the serum LH levels of castrated male goats (P＜001),and these effects were blocked by the opioid antagonist naloxone.The serum FSH levels of male goats had no significant changes(P＞005) before and after ruminal infusions of βcasomorphin.
出处
《安徽农业大学学报》
CAS
CSCD
北大核心
2003年第1期66-69,共4页
Journal of Anhui Agricultural University
基金
国家自然科学基金项目(39870569)
安徽省自然科学基金项目(98130707)资助。
