摘要
背景及目的:本研究拟通过对卵巢上皮性肿瘤患者血清血管内皮生长因子(vascularepithelialgrowthfactor,VEGF)水平的检测及对卵巢上皮性肿瘤组织中VEGF表达的观察,以探讨VEGF与卵巢上皮性肿瘤临床、病理诸因素的关系。方法:卵巢上皮性肿瘤患者73例,其中24例为良性肿瘤患者(良性组),7例为交界性上皮瘤患者(交界性组),42例为恶性肿瘤患者(恶性组);以同期在本科室查体无明显妇科疾患的20名妇女血清做对照。用免疫组化二步法检测73例卵巢上皮性肿瘤患者肿瘤组织中VEGF的表达,用ELISA法测定71例卵巢上皮性肿瘤患者术前血清及10例患者腹水中VEGF水平,对其中7例卵巢上皮性癌患者血清在术后进行了定期检测。结果:①VEGF在卵巢上皮性肿瘤组织中的表达,恶性组阳性表达率(86.36%)明显高于交界性组(66.67%,P<0.005)及良性组(37.50%,P<0.005)。②良性组、交界性组及恶性组之间血清VEGF水平的差异有统计学意义(P<0.01);在恶性组中,血清VEGF水平在临床Ⅲ、Ⅳ期明显高于Ⅰ、Ⅱ期(P<0.05),在病理分化差的G3组明显高于病理分化好的G1、G2组(P<0.01),而与病理类型相关性无统计学意义;卵巢癌患者经理想的肿瘤细胞减灭术后,血清VEGF水平均有不同程度下降,有3例患者病情复发后,血清VEGF水平再度上升。
BACKGROUND &OBJECTIVE:This study was desig ned to examine the level of serum vascular e pithelial g rowth factor (VEGF )and VEGF expression in the patients with ovar ian epithelial carcinoma (EOC)and to evaluate the relationship between V EGF and the clinicopatholog ical factors.METHODS :Seventy-three patients with ovaria n epithelial tumor were selected as study g roup,including 24benig n epi thelial ovarian tumor (BET),7borderline epithelial ovarian tumor (BOT ),42EOC.Twenty women without obvious g ynecolog ic diseases were selected as serum control g roup(CON).VEGF expression in the epithelial ovaria n tumor tissues from 73patients were analyzed immunohistochemically.Serum VEGF in 71patients and ascite VEGF in 10patients were detected by enzyme-li nked immunosorbent assay(ELISA)and 7cases with EOC were continuously det ermined after operation.RESULTS :(1)Positive immunostaining of VEGF was observed in 86.3%of EOC,which was sig nificantly hig her than that of BO T(66.67%,P<0.005)and BET(37.5%,P<0.005).(2)There were sig nificant differences in serum VEGF level among BET,BOT,and EOC g roup(P<0.01).Serum VEGF levels before operation in patients with EOC were sig nificantly hig her than those with BOT,BET,and CON(P<0.01).Serum VEGF levels in EOC patients at advanced stag e (stag e Ⅲor Ⅳ),low differentiation(G 3 )were apparently hig her than those at early stag e (stag e Ⅰor Ⅱ)(P<0.05)and hig h differentiation(G 1 ,G 2 )(P<0.01),respectively.Serum VEGF levels decreased after the successful removal of tumor in ovarian cancer patients.The seru m VEGF levels were re-elevated durin g relapse in three patients.CONCLUSION:(1)The elevated serum VEGF levels and increased VEGF expression in EOC were associated with its malig ment behavior.(2)Serum VEGF could be used as a marker fo r monitoring the clinical course of the patients with epitheli al ovarian carcinoma.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2003年第1期58-61,共4页
Chinese Journal of Cancer
关键词
ELISA
卵巢上皮性肿瘤
血清
肿瘤组织
VEGF
临床意义
Epithelial ovarian tumor
Vascular epithelial g rowth factor (VEGF )
Enzyme-linked immunosorbent assa y(ELISA)
Immunohistochemistry.
