摘要
沙葱 (AlliummongolicumRegel)的叶基切段作外植体 ,成功地诱导出愈伤组织和再生植株。诱导愈伤组织的培养基为附加 2mgL- 1 2 ,4-D和 0 .2mgL- 1 KT的MS培养基。愈伤组织转移到附加 2mgL- 1 6BA和 0 .4mgL- 1 NAA的MS培养基后分化出大量的苗。分化苗在含有IBA 1mgL- 1 的 1 /2MS培养基上生根最佳。再生植株移栽成活率在 95 %以上 ,移栽到田间的植株第二年以后可正常开花结实。
A highly efficient regeneration system of Allium mongolicum Regel was established from leaf base segments via tissue culture. MS medium supplemented with 2mgL -1 2,4-D and 0.2mgL -1 KT was suitable for callus induction. The shoots differentiated on MS medium added with 2mgL -1 6BA and 0.4mgL -1 NAA. The half strength MS medium containing 1mgL -1 IBA was more effective for the inducing roots. The regenerated plantlets were transplanted to the soil, and over 95% of them were survival. At the second year after transplanting, the plantlets flowered and set seeds.
出处
《植物研究》
CAS
CSCD
北大核心
2003年第1期51-54,T010,共5页
Bulletin of Botanical Research
基金
国家自然科学基金 (批准号 30 0 70 36 6 )资助
