摘要
利用分葱茎尖培养成功地诱导了体细胞胚胎发生。具体步骤如下：Ⅰ．在ＭＳ＋２，４－Ｄ１ｍｇ／Ｌ＋ＢＡ２．０ｍｇ／Ｌ培养基上诱导愈伤组织；Ⅱ．在ＭＳ＋２，４－Ｄ０．５ｍｇ／Ｌ＋ＢＡ２．０ｍｇ／Ｌ培养基上进行愈伤组织的继代培养，继代培养３次以后，转变为胚性愈伤组织；Ⅲ．在ＭＳ＋２，４－Ｄ０．２５ｍｇ／Ｌ＋ＢＡ２．０ｍｇ／Ｌ培养基上进行胚状体的诱导；Ⅳ．在无任何激素的ＭＳ培养基上使胚状体再生植株。切片观察表明，胚状体大多数起源于愈伤组织表层及其以下细胞。
Somatic embryogenesis on bunching onion could be induced as the following processes:Ⅰ. The callus was induced on MS+2,4-D 1.0mg/L+BA 2.0mg/L. Ⅱ. The callus was subcultured on MS+2,4-D 0.5mg/L+BA 2.0mg/L and after 3 times embryogenic callus could be obtained. Ⅲ. Embryoids were induced on MS+2,4-D 0.5mg/L+BA 2.0mg/L. Ⅳ. Plantlets regenerated on MS media with no growth regulators.Morphological studies showed that most embryoids originated from the surface callus cells and the sub layer cells.
出处
《园艺学报》
CAS
CSCD
北大核心
1997年第3期264-268,共5页
Acta Horticulturae Sinica
基金
山东省自然科学基金
关键词
分葱
愈伤组织
胚状体
体细胞胚
Bunching onion
Callus
Embryoid
Somatic embryogenesis
