摘要
[目的 ]通过观察金雀异黄素 (genistein ,GS)、大豆苷元 (daidzein ,DA)和大豆黄素 (glycitein ,GL)对前列腺癌细胞 (PC 3 )增殖的影响 ,探讨通过膳食干预途径预防前列腺癌发病的可行性。 [方法 ]将PC 3在PRMI 164 0培养液 (含10 %小牛血清 )中采用开放式单层贴壁培养。实验设溶剂对照组、顺铂阳性对照组及三种受试物各三个剂量组 (5× 10 -6mol/L ,2 5× 10 -6mol/L ,75× 10 -6mol/L) ,采用MTT法、3 H TdR掺入法及流式细胞术对PC 3的增殖情况进行分析。 [结果 ]与溶剂对照组相比 ,2 5 μmol/LGS、75 μmol/LDA和 75 μmol/LGL对PC 3处理 72h可抑制PC 3增殖 ,抑制率分别为42 %、5 0 %及 3 9%。三种受试物均可抑制细胞DNA合成并推进G0 /G1期细胞进入S期 ,降低细胞增殖指数。 [结论 ]大豆异黄酮类化合物GS、DA和GL均具有明显抑制前列腺癌细胞PC 3增殖效应 ,并呈现剂量 效应和时间 效应关系 。
To explore the feasibility of dietary intervention through resesrch on effects of flavonoid on the proliferation of prostate cancer cells (PC 3). Prostate cancer cells were grown in RPMI 1640 medium supplemented with 10% bovine serum and 5 ml 10 000 U/ml of penicillin/streptomycin in an incubator maintained at 5% CO 2 and 37℃. The respective test compound was added into fresh medium,the control cells received only the vehicle(dimethyl sulfoxide,DMSO).The proliferation of PC 3 cells was analyzed by MTT assay, 3H TdR incorporation assay and flow cytometry. Compared with the DMSO control,this study demonstrated that the proliferation and DNA synthesis of prostate cancer cells treated with genistein(25 mmol/L,72 h),daidzein,(75 mmol/L,72 h) and glycitein (75 mmol/L,72 h) respectively were markedly inhibited.The results indicated that there is a time dependent and dose dependent relationship. [Conclusion] Soy isoflavones (genistein,daidzein,glycitein) could significantly inhibited the proliferation of PC 3 cells.The findings give a support to the hypothesis that dietary isoflavones can prevent the incidence of prostate cancer.
出处
《环境与职业医学》
CAS
北大核心
2002年第6期352-354,共3页
Journal of Environmental and Occupational Medicine
基金
国家自然科学基金重点课题 (编号 :30 0 30 1 2 0 )
