摘要
目的:探讨两种大豆异黄酮主要成分染料木黄酮(genistein,GEN)和大豆苷元(daidzein,DAI)抑制人乳腺癌MCF-7细胞增殖的作用与过氧化物酶体增殖激活物受体γ(peroxisome proliferators-activated receptorγ,PPARγ)信号途径的关系。方法:采用免疫细胞化学染色方法观察MCF-7细胞的PPARγ表达情况,PPARγ介导的荧光素酶报告基因检测大豆异黄酮和PPARγ配体罗格列酮(rosiglitazone,ROS)对MCF-7细胞PPARγ的激活作用,MCF-7细胞分别经8×10-5mol/L GEN、DAI和1×10-5mol/L的ROS单独或联合1×10-5mol/L的PPARγ特异性抑制剂GW9662联合处理24、48和72 h后,用CCK-8法检测细胞增殖。结果:MCF-7细胞存在有PPARγ表达,GEN、DAI呈剂量依赖性增强报告基因荧光素酶活性,且这种作用可被GW9662明显阻断;GEN、DAI和ROS呈时间依赖性明显抑制MCF-7细胞增殖(P<0.05),而GW9662可以显著削弱GEN、DAI和ROS对MCF-7细胞的增殖抑制作用(P<0.05)。结论:大豆异黄酮可通过激活乳腺癌MCF-7细胞的PPARγ信号途径抑制其增殖。
Objective：To investigate the effect of two major components of soy isoflavones genistein,daidzein and peroxisome on the proliferation of human breast cancer MCF-7 cells,and the relationship between it and the proliferators-activated receptor γ（PPARγ）signal pathway.Methods：The expression of PPARγ in breast cancer MCF-7 cells was detected by immunocytochemical staining,and the activation of PPARγ in MCF-7 cells induced by isoflavones was measured by luciferase reporter gene assay.The proliferation of MCF-7 cells was measured by CCK-8 assay.Results：There was expression of PPARγ in breast cancer MCF-7 cells.The luciferase ac-tivities of MCF-7 cells tranfected with luciferase reporter gene plasmid showed a dose-response relationship with genistein and daidzein treatment.GW9662 significantly inhibited the expression of reporter gene induced by genistein and daidzein.Genistein,daidzein and rosiglitazone inhibited the proliferation of cancer cells MCF-7in a time-dependent manner（P0.05）,but the inhibitory effects of genis-tein,daidzein and rosiglitazone on the proliferation of cancer cells could be blocked by GW9662 significantly（P0.05）.Conclusions：Soy isoflavones inhibit the proliferation of breast cancer MCF-7 cells by activating the PPARγ signal pathway.
出处
《现代生物医学进展》
CAS
2011年第6期1025-1029,共5页
Progress in Modern Biomedicine
基金
国家自然科学基金(No.30771793)
