摘要
采用碳二亚胺法制备诺氟沙星与牛血清白蛋白(BSA)的结合物,作为诺氟沙星酶联免疫用的免疫原。经紫外光谱法测定,每分子牛血清白蛋白连接的诺氟沙星分子数为16.1个。分别用碳二亚胺法和混合酸酐法制备诺氟沙星与卵清蛋白(OA)的结合物,作为诺氟沙星酶联免疫测定用的包被抗原。经紫外光谱法测定,每分子卵清蛋白连接的诺氟沙星分子数分别为5.8和1.9个。
The antigenic conjugate of norfloxacin and bovine serum albumin ( BSA) was prepared by carbodiimide method. 16. 1 norfloxacin residues were linked to a molecule of the BSA in the conjugate by method of UV spectrum. The conjugates of norfloxacin-ovalbumin (OA) used for coating antigen were synthesized by carbodiimide method and mixed anhydride method, respectively. The number of norfloxacin residues per molecule of the conjugates was 5 .8 and 1.9.
出处
《上海水产大学学报》
CSCD
2002年第4期362-366,共5页
Journal of Shanghai Fisheries University
