摘要
以 2 ,3-二氢 - 2 ,2 -二甲基 - 7-苯并呋喃酚为反应原料 ,在通光条件下合成了 2 ,3-二氢 - 2 ,2 -二甲基 - 7-苯并呋喃基氯甲酸酯 .然后 ,以其作为反应原料与氨基已酸或氨基丁酸反应 ,在不同条件下合成了具有羧基的两种活性半抗原 6- [〔(2 ,3-二氢 - 2 ,2 -二甲基 - 7-苯并呋喃基氧 )羰基〕氨基 ]已酸 (BFNH)和4 - [〔(2 ,3-二氢 - 2 ,2 -二甲基 - 7-苯并呋喃基氧 )羰基〕氨基 ]丁酸 (BFNB) .半抗原和牛血清蛋白 (BSA)经碳二亚胺法偶联得到了其免疫原 ,BFNH- BSA和 BFNB- BSA的结合比分别为 10∶ 1和 13∶ 1,同时利用混合酸酐法和卵清蛋白经偶联得到包被抗原 ,BFNH- OVA和 BFNB- OVA的结合比分别为 4∶ 1和3∶
This study reported that the first compound, 2,3 dihydro 2,2 dimethyl 7 benfuranyl chloroformate (DDBC) was synthesized after using 2,3 dihydro 2,2 dimrthyl 7 benzofuranol reacted with CoCl 2. Then two haptens named 4 [〔(2,3 dihydro 2,2 dimethyl 7 benfuranyloxy) carbonyl〕 amino] hexanoic acid(BFNH) and 6 [〔2,3 dihydro 2,2 dimethyl 7 benfuranyloxy) carbonyl〕 amino] butanoic acid(BFNB) were synthesized with the reaction between DDBC and amino hexanoic acid or amino butanoic acid under different conditions. The BFNH and BFNB were conjugated to bovine serum albumin (BSA) with the modified active ester method to form two antigens for producing the high titre antibodies to carbofuran. The ratios of BFNH BSA and BFNB BSA were 10∶1 and 13∶1, respectively. Other two coating antigens for carbofuran, BFNH OVA and BNFB OVA were also synthesized by conjugating BFNH or BFNB to ovalbumin (OVA) with the mixed anhydride method, and their ratios were 4∶1 and 3∶1, respectively.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2002年第1期47-53,共7页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
浙江省科技厅资助项目 ( 98110 2 3 14 )
