摘要
本文首次证实小鼠肉瘤180(S_(180))细胞可在体内外自发分泌高滴度的粒细胞集落刺激因子(GCSF)。分泌活性持续稳定。LPS、PHA可刺激S_(180)产生高分泌活性。咐波豆冠酸乙酯(TPA)具有协同作用;钙离子通透剂A23181在TPA协同下有很强的诱导作用。ConA无刺激S180分泌GCSF作用,并可抑制TPA的诱导活性.S180的腹水中亦含有高滴度的GCSF活性。腹水瘤细胞转入体外培养后仍保持GCSF分泌活性,为大量制备、纯化S180GCSF提供了有利的条件。
Our results demonstrated definitely that S180 cells produce GCSF in vitro and in vivo either constitutively or stimulatively by LPS and PHA but not by ConA. Higher activity of GCSF could be stimulated by the combined use of A23187 and TPA
出处
《第一军医大学学报》
CSCD
1992年第2期106-109,共4页
Journal of First Military Medical University
