摘要
作者设计并合成了一对位于HIV-1pol基因、保守性很高的寡核苷酸引物,采用3温度点及2温度点聚合酶链式反应(PCR),以pARV-2/7A质粒为模板,扩增出360bp大小的片段.以pUC 19,pTTQ 18,M13mp18和外周血淋巴细胞(PBMCs DNA)为模板进行同步PCR,并用倍比稀释法将pARV-2/7A稀释后进行PCR,分别研究了该组引物的特异性和敏感性.结果表明:该套PCR试剂特异性强,敏感度高,能检出3个拷贝的HIV-1.
With two oligonucliotide primers, HTV-1 Pr 1 and HPV-1 Pr 2, authors amplified a highly conservative sequence ( 360 bp long) of human immunodeficiency virus type 1 (HIV-1) pol gene by using three- and two-tern perature-point polymerase chain reaction (PCR). The templates were pARV-2/7A DNA which contained HIV-1 whole gene; pUC 19, pTTQ 18, M13mpl8 and PBMCs DNA. The results showed that HIV-1 protaese gene was just only specifically amplified from pARV-2/7A, and we could detect three molecules of HIV- 1 gene from the samples in 30 min by two-temperature-point PCR. Based on the results of our experiment it is concluded that this PCR system are usable in clinical detection of HIV.
出处
《第四军医大学学报》
1992年第6期408-410,共3页
Journal of the Fourth Military Medical University
关键词
聚合酶链反应
基因
艾滋病毒
polymerase chain reaction
human immunodeficiency virus
gene
