摘要
艾滋病相关病毒(ARV-2)是目前应用最广的艾滋病毒分离株之一.其基因组大小为9737碱基.为便于研究工作,作者对其进行了亚克隆.用Kpn I消化pARV-2/7A产生4个DNA片段,其中含有env基因和pUC19序列的大片段自身环化,得到亚克隆质粒pJE332.其余3个片段分别与pUC19连接,得到另3个亚克隆质粒pJG423,pJP 032和pJP163.其中pJG423含有LTR序列、gag基因和部分pol基因,pJp032和pJP163均插入了部分pol基因.这些亚克隆质粒已被用于各项艾滋病研究.
We introduced the cDNA clone of ARV-2 from USA in 1988. We subcloned this full-length cDNA into pUC19 by Kpn I site. Digested with Kpn I, the recombinant plasmid pARV-2/7A was cleaved into 4 fragments. The 6.0 kb fragment was recircularized, and the other three fragments were ligated with pUC19 plasmid cut open by Kpn I. By screening and identification, four subclones were obtained. Of which, pJE332 contains the whole env gene, pJG423 contains LTR, gag and part of pol gene, pJP163 and pJP032 contain only part of pol gene. These subclone plasmids have been being used in many investigations on gene detection and cloning of HIV type I.
出处
《第四军医大学学报》
1992年第4期264-267,共4页
Journal of the Fourth Military Medical University
关键词
免疫缺陷病毒
基因
亚克隆
质粒
human immunodeficiency virus
AIDS associated retrovirus
gene
subcloning
restriction enzyme
plasmid
