摘要
作者以琼脂免疫扩散试验和免疫斑点法,对临床分离的金黄色葡萄球菌58株通过产毒培养产生的不同血清型的肠毒素进行了鉴定.两法检出的阳性率分别是62.0%(36/58)和77.5%(45/58),前法以检出单一型肠毒素的菌株占多数(24/36),尤以A型居多(21/36);后法则以检出1型以上(多型)肠毒素占多数(34/45),亦以A型出现频率最高(42/45).结果提示,本地区葡萄球菌的产毒率比较高,且以A型肠毒素最为常见.
A various serum types of enterotoxins produced by 58 Staphylococcus aureus strains isolated from clinical specimens via.culture on enterotoxigenic media have been identified by both agar immunodiflusion test (AIT) and dot-imrnunobinding assay (DIA). The results showed that the positive percentages detected by both methods were 62.0% (36/58) and 77.5% (45/58) respectively. 24/36 enterotoxigenic strains produced only a single enterotoxin in the AIT, and 34/45 enterotoxigenic strains, produced more types in DIA. It indicates that enterotoxigenesis rate of S.aureus strains isolated from our area is higher, and SEA-producing strains among these are mostly frequent.
出处
《第四军医大学学报》
1992年第5期356-358,共3页
Journal of the Fourth Military Medical University
关键词
葡萄球菌
肠毒素
血清型
免疫学
staphylococcal, enterotoxin
serum type
immunologic method