摘要
目的通过检测龈沟液(GCF)中Ⅱ型胶原酶(Ⅱtypecollagenase,COL-Ⅱ)、天冬氨酸转氨酶(aspartateaminotransferase,AST)、细胞外弹性蛋白酶(EAinthesupernatant,EA-s)的水平,探讨三种酶作为早期牙周病诊断指标的可行性。方法用滤纸条的袋口取样法取90例患者的GCF样本,分别采用ELISA法测定COL-Ⅱ水平,罗氏全自动生化检测仪测定AST水平,底物法测定EA-s水平。结果COL-Ⅱ、AST、EA-s水平在牙周炎组、牙龈炎组、正常组之间均有显著差异(P<0.001),酶水平与龈沟液体积、探诊深度、附着丧失、龈沟出血指数呈正相关关系,三种酶之间存在正相关关系。结论龈沟液中的COL-Ⅱ、AST、EA-s水平对早期牙周病的辅助诊断有较大参考价值。
Objective To determine the possibility of type collagenase(COL- ), aspartate aminotransferase(AST), elastase in the supernatant(EA-s) in gingival crevicular fluid(GCF) as the diagnostic parameters for early periodontal disease through determining their levels in GCF. Methods GCF was sampled with filter strips by entrance-pocket method from ninty patients. The levels of COL- , AST and EA-s were determined separately by ELISA, Roche automatic biochemistry analyzer and the substrate Meosuc-ala-ala-pro-val-NA. Results The levels of COL- , AST, EA-s were significantly different among periodontitis, gingivitis and healthy group(P<0.001). The GCF volume, probing depth, attachment loss and sulcus bleeding index were positively related to the levels of enzymes. There were also positive relationships among these three enzymes. Conclusions COL- , AST, EA-s could be helpful in early diagnosis for periodontal disease.
出处
《上海口腔医学》
CAS
CSCD
2002年第4期294-296,共3页
Shanghai Journal of Stomatology
关键词
龈沟液
早期
牙周病
诊断
胶原酶
天冬氨酸转氨酶
胰肽酶
Gingival Crevicular Fluid
Periodontal Disease
Collagenase
Aspartate Aminotransferase
Pancreatopeptidase
