摘要
作者以胶体金标记抗流行性出血热病毒多克隆及单克隆抗体的直接免疫金银法,做流行性出血热尸检、活检及动物组织中病毒抗原的免疫细胞化学定位,并同ABC,PAP及APAAP法进行比较。该法操作简便,金标一抗与组织孵育后即可显色,所需时间较上述对比方法缩短2～4h,并能定位长期保存尸检组织中,用上述对比方法难以检测到的病毒抗原。该法可不受组织内色素、内源酶活性及交叉染色对结果的干扰,是一种较理想的免疫细胞化学染色方法。
: Direct immunogold - silver staining (D - IGSS )using gold labeling rabbit polyclonal antibody (PAb )and monoclonal antibodies (MAb )to EHFV were employed in the immunocytochemical localization of viral antigens in the EHF tissues . Compared with PAP , ABC and APAAP method , in all the cases , the D - IGSS method was found to give superior or at least equal results to those obtained with PAP , ABC and APAAP methods . and could also demonstrate the viral antigens in the long time keeping autopsy tissues when those methods gave no or weak staining . The D - IGSS method was much easier to perform and time saving . It had the sensitivity of IGSS , and was little affected by the pigments and endogenous peroxidase activity in the tissues . The D - IGSS method is a valuable technique in the detection of viral antigens in the EHF tissues .
出处
《第四军医大学学报》
1992年第1期29-31,共3页
Journal of the Fourth Military Medical University
关键词
出血热
流行性
免疫金银技术
抗原
hemorrhagic fever, epidemic
immunocytochemistry
immunogold - silver staining
