摘要
应用抗人增殖细胞抗体Ki-67,以ABC法对15例恶性黑素瘤(MM)、17例后天性痣、18例先天性小痣及3例先天性巨痣进行标记染色。结果显示:13例MM阳性(细胞阳性率2％～33％),3型色素痣全部阴性。培养的MM细胞阳性率88％。结果提示:就黑素细胞肿瘤而言,Ki-67是鉴别良、恶性的一个较敏感和特异的指标;目前用Ki-67这种直接反应肿瘤增生活性的方法尚不足以反映不同型良性黑素细胞肿瘤之间存在的微小差别。
Fifteen cases of malignant melanoma,17 cases of acquired nevus,18 cases of congenital small nevus,and 3 cases of congenital giant nevus were labeled with anti-human prolifera-tive cell antibody Ki-67 with ABC labeling method.It was found that 13 out of the 15 cases of malignant melanoma were positive and the positive rate of the tumor cells was ranged between 2% to 33%.After culture,the positive rate of the melanoma cells was 88%.But all the 3.types of melanocytic nevi were negative.The findings suggest that the labeling of anti-human proliferative cell antibody Ki-67 is a sensitive and specific method to identify melanocytic tumors and it is not sensitive enough to differentiate the minor changes of the 3 types of nevi.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1992年第5期427-429,共3页
Journal of Third Military Medical University
基金
国家自然科学基金资助项目
关键词
黑色素瘤
抗体
melanoma
melanocytic nevus
Ki-67
