摘要
本文利用大鼠肝细胞核体外转录体系,探讨放线菌素D对染色质(或DNA)模板的抑制效应,并探讨人工合成的PolyC〔d(A-T)〕的转录模板效应;在此基础上测定细胞核游离型RNA聚合酶(RNA Pol)的活性,对以高渗(2.3mol/L)蔗糖一次提取的正常大鼠肝细胞核,比较了其中两类不同功能状态的RNA Pol的相对含量,并对游离型RNA Pol在真核细胞RNA转录过程中的作用进行了讨论。
The inhibitory effects of actinomycin D on chromatin (or DNA) template and the stimulating effects of synthetic template Poly [d(A-T)] on transcription were observed in the liver nucleic transcriptional system of the rat in vitor.By introducing proper concentrations of actinomycin D and Poly [d(A-T)] into each reactionary system,a method to assay free RNA polymerase activity in the nucleus was established.In addition,a comparison of the contents between chromatin-engaged and free polymerase in normal rat liver nuclei isolated with hypertonic sucrose (2.3 mol/L) was made.Eventually,the possible role free RNA Polymerases play in the eukaryotic transcriptional process was discussed.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1992年第3期216-219,共4页
Journal of Third Military Medical University
关键词
核糖核酸
聚合酶类
转录
遗传
大鼠
RNA Polymerases
transcription
genetic
rat
actinomycin
