摘要
目的:研究血管紧张素原(AGT)基因T174M和M235T多态性与高血压并发冠心病的相关性。方法:运用单管双相等位基因专一性扩增(single鄄tubebi鄄directionalallelespecificamplification,SB鄄ASA)方法,在中国人群中,对115例冠心病合并高血压病人、97例高血压对照者和90例正常对照者进行T174M和M235T基因分型。结果:M235T多态C/C、C/T和T/T基因型在冠心病合并高血压组中的分布与正常对照和高血压对照相比差异均有显著性(P<0.01);C、T等位基因频率与对照组相比差异也有显著性(分别为P<0.05,P<0.01)。但在冠心病合并高血压组中,M235T的分布差异与冠状动脉粥样硬化病变程度无相关性。结论:AGT基因M235T多态性可能是中国高血压病人并发冠心病的一个遗传性危险因素。
Objective:To evaluate the correlation between T174M and M235T polymorphism o f angiotensinogen(AGT)gene and coronary heart disease(CHD)complicated by essenti al hypertension(EH)in Chinese population.Methods:Single-tube bi-directional al lele specific amplification(SB-ASA)was used to genotyping T174M and M235T in115p atients both with CHD and EH,97patients with EH and90normal controls.Results:The C/C,C/T and T/T genotypes of M235T poly-morphisms were significantly different among group of CHD complicated by EH,group of EH and group of normal controls(P <0.01).Frequencies of C and T alleles were also different in these groups(P<0.05 and P<0.01respectively).But in group of CHD complicated by EH,the distribution d ifference of M235T was not correlated with the extent of coronary atherosclerosi s.Conclusions:M235T polymorphism of AGT gene might be a genetic risk factor of C HD in Chinese pa-tients with EH.
出处
《诊断学理论与实践》
2002年第4期231-233,236,共4页
Journal of Diagnostics Concepts & Practice
基金
上海市科技发展基金(No.974119003)
