摘要
目的 构建既具有活性血管平滑肌细胞 (VSMC)中膜层 ,又有血管内皮细胞 (VEC)内皮化的复层结构组织工程血管支架材料。方法 1将聚羟基乙酸 (PGA)纤维无纺网、VSMC和胶原纤维相混合 ,设计构建 VSMC活性的组织工程血管支架材料 ;2采用酶消化法从兔主动脉中分离培养兔 VEC并传代、纯化 ,接种于组织工程人工血管支架的内腔 ,体外培养 ,并行电镜观察。结果 构建的支架材料具有一定弹性和韧性 ,VEC在其内表面形成较完整内皮细胞层 ,且 VSMC能够保持活性 ,生长状况良好。结论 经胶原包埋处理的 PGA支架可作为组织工程化人工血管研究较理想支架材料 ;为组织工程方法构建具有分层结构的组织工程血管奠定实验基础。
Objective To investigate the feasibility to seed vascular endothelial cell(VEC) and vascular smooth muscle cell (VSMC) into tissue engineered blood vessel scaffold material. Methods ①A blood vessel scaffold with a combined polymer was designed, which mainly is composed of rabbit VSMC and collagen with reinforcement by a non spinning fabric mesh made of polyglycolic acid (PGA). ② VEC were isolated from rabbit thoracic aorta by enzyme digestion methods and subcultured and purified. Then the cells were seeded into scaffold material. The morphological characteristics of tissue engineered blood vessel was analyzed by scanning electron microscopy. Results VEC could adhere well to the inner surface of the tissue engineered tubular scaffold material with a tenacity and elasticity. VSMC could sustain bioactivity of cell. Conclusion Non spinning PGA porous biodegradable materials coated with collagen is benefit for cells to adhere and grow. It will lay a foundation of a laminated structure of tissue engineered blood vessel.
出处
《中国修复重建外科杂志》
CAS
CSCD
2003年第1期65-68,共4页
Chinese Journal of Reparative and Reconstructive Surgery
基金
国家自然科学基金资助项目 (30 0 70 777)
