摘要
目的 :观察在高血压不同时期心肌胶原网络的改变 ,为高血压的治疗及心脏功能的早期保护提供基础研究的依据。方法 :采用Masson三色法染色光镜观察和天狼星红 苦味酸饱和液染色偏振光显微镜观察两种方法 ,对 8周、1 2周、2 4周自发性高血压大鼠心肌胶原进行观察 ,采用计算机图象分析方法 ,对高血压不同时期心肌胶原总量和Ⅰ /Ⅲ型胶原比例的改变进行观测。结果 :1 2周时心肌胶原总量较 8周时增多 ,Ⅰ /Ⅲ型胶原量比例有轻度增加。2 4周时 ,心肌胶原增生明显 ,局部出现心肌纤维化 ,胶原网络构筑杂乱无章。血管周围胶原增生更为显著 ,且向周围心肌间质中大面积蔓延。Ⅰ型胶原增多 ,Ⅰ /Ⅲ型胶原量比例增大。结论 :在高血压时 ,心肌胶原网络重建 ,与病程关系密切 ,病程越长 。
Objective:To study the changes of myocardial collagen network at different stages of the spontaneous hypertension,providing a new way for curing hypertension and the early heart protection. Methods:With the Masson staining with optical microscope and Sirius Red in a supersaturated picric acid solution staining with polarized microscope,myocardial collagen of the spontaneous hypertensive rats (SHR) about the modality and structure were observed in the 8th week,the 12th week and the 24th week.By using image analysis,the changes of the total myocardial collagen and the rate of type Ⅰ collagen to type Ⅲ were analyzed. Results:In the 12th week the total collagen increased more than in the 8th week,the rate of type Ⅰ collagen to type Ⅲ had a small increase. In the 24th week,the collagen increased greatly,myocardial fibrosis raised in some parts,the collagen network was irregular. The collagen had a big increase around the vascular and spreaded out to myocardium.the rate of type Ⅰ collagen to type Ⅲ was increased. Conclusion:As the hypertension course lasts the myocardial collagen network is remodeled,the change is very serious when the time is very long.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2002年第5期432-436,共5页
Chinese Journal of Anatomy
基金
北京大学基础医学院凯华基金赞助
